Introduction and Objectives: The ‘One Stop Prostate Clinic’ (OSPC) was a same day prostate cancer assessment and diagnostic clinic at a public tertiary hospital in Australia. Eligible men were offered the option to receive initial notification of their biopsy results via telephone call from the OSPC Clinical Nurse (CN). The preferences and experiences of those men who consented to and received initial telephone notification of their prostate biopsy results by the OSPC Clinical Nurse (CN) are reported. Methods: Prospective mixed method study using survey instrument and thematic analysis of the preferences and experiences of men attending the OSPC. Eligible men provided their verbal consent to receive telephone notification of their prostate biopsy results. Men were ineligible if they either required an interpreter or insufficient proficiency with English or were a prisoner. Men were informed of their biopsy results (cancer or benign), Multi-Disciplinary Team (MDT) discussion, prostate cancer resources (if applicable) and follow up arrangements by the OSPC CN. Results: 1,000 men attended the OSPC between August 2011 and November 2017 and 876 underwent prostate biopsies. 791/876 (90%) men verbally consented to receive telephone notification of biopsy results by the OSPC CN, 6/876 (1%) men declined and 79/876 (9%) men were ineligible/not contacted. Between December 2015 and October 2018, 220/403 men (55%) returned the survey instrument (OSPC questionnaire). 135/220 (61%) men received a cancer diagnosis and 85/220 (39%) men had benign pathology. Of those men with a cancer diagnosis, 119/132 (90%) men would choose this method again, 7/132 (5.5%) men would not and 6/132 (4.5%) men were unsure. 94/135 (70%) men reported no disadvantages with this notification method. Overall satisfaction rate with the OSPC was 96% (209/218) men. Thematic analysis of written comments - 51 positive comments related to professional conduct and 10 negative comments related to communication issues. Conclusion: Nurse led initial telephone notification of prostate biopsy results by the OSPC CN was preferred by the vast majority of eligible men. Many men with a cancer diagnosis did not experience any disadvantages and would choose this method again. This approach could easily be incorporated into routine clinical practice by other tumour groups.