Based on the micro-bubbling process as an intensification method, an experimental study on hydroformylation of 1-hexene was carried out. Effects of different total pressures, temperatures, partial pressures and substrate concentrations on the conversion rate of olefin and normal-to-iso ratio were investigated and the reaction kinetic coefficients were determined by fitting the experimental data with the model. Using the Euler two-fluid and standard k-ε turbulence model, a computational fluid dynamics model was established to describe the multi-phase flow mass transfer and reaction of olefin hydroformylation. The simulation results were in good agreement with the experimental results. Furthermore, the effects of microbubble sizes and internal components on the overall gas holdup, mass transfer rate, turbulent mixing and reaction efficiency of the multi-phase 1-hexene hydroformylation reaction system were studied, which could be applied to the process intensification and scale-up of the various multi-phase gas-liquid hydroformylation reactions.