Effects of Multiple Near-Infrared LEDs (700, 850, and 980 nm) CW-PBM on Mitochondrial Respiration and Gene Expression in MG63 Osteoblasts
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Hryciw, Deanne H
Walsh, Laurence J
Ranjit, Eliza
Tomy, Nifty
Arany, Praveen R
George, Roy
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BACKGROUND AND AIM: This study evaluated mitochondrial and osteogenic activity in MG-63 pre-osteoblastic cells after photobiomodulation (PBM) using multiple near-infrared LED sources (Nuralyte) emitting wavelengths from 700 to 1100 nm. MATERIALS AND METHODS: MG-63 cells were irradiated daily for 3, 5, or 7 days with energy densities of 5.3 J/cm2 (30 s, optimal dose) and 10.6 J/cm2 (60 s, high dose). Mitochondrial function was assessed using the XF Seahorse analyzer, and gene expression of osteogenic markers was analyzed. RESULTS: Maximal mitochondrial oxygen consumption rate (OCR) significantly decreased at the optimal dose but increased at the high dose (p < 0.001) in 5-day irradiated cultures. Upregulation of osteogenic markers (OCN, OPN, BMP-2, COL-1, RUNX2) occurred after 3-5 consecutive days of irradiation, with greater activation at the optimal dose. CONCLUSION: MG-63 cells respond to PBM using MNI-LEDs (700, 850, 980 nm) by modulating mitochondrial respiration and boosting bone-related gene expression in a dose- and time-dependent manner.
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Journal of Biophotonics
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© 2025 The Author(s). Journal of Biophotonics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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Analytical chemistry
Medicinal and biomolecular chemistry
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Sleep, S; Hryciw, DH; Walsh, LJ; Ranjit, E; Tomy, N; Arany, PR; George, R, Effects of Multiple Near-Infrared LEDs (700, 850, and 980 nm) CW-PBM on Mitochondrial Respiration and Gene Expression in MG63 Osteoblasts, Journal of Biophotonics, 2025, pp. e70015