Simultaneous identification of Haemophilus influenzae and Haemophilus haemolyticus using real-time PCR
File version
Version of Record (VoR)
Author(s)
Harris, Tegan M
Spargo, Jessie C
Nosworthy, Elizabeth
Beissbarth, Jemima
Chang, Anne B
Smith-Vaughan, Heidi C
Sarovich, Derek S
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
Size
File type(s)
Location
Abstract
Aim: To design a highly specific and sensitive multiplex real-time PCR assay for the differentiation of the pathogen Haemophilus influenzae from its nonpathogenic near-neighbor Haemophilus haemolyticus. Materials & methods: A comparison of 380 Haemophilus spp. genomes was used to identify loci specific for each species. Novel PCR assays targeting H. haemolyticus (hypD) and H. influenzae (siaT) were designed. Results & discussion: PCR screening across 143 isolates demonstrated 100% specificity for hypD and siaT. These two assays were multiplexed with the recently described fucP assay for further differentiation among H. influenzae. Conclusion: The triplex assay provides rapid, unambiguous, sensitive and highly specific genotyping results for the simultaneous detection of hypD and siaT, including fucose-positive H. influenzae (fucP), in a single PCR.
Journal Title
Future Microbiology
Conference Title
Book Title
Edition
Volume
12
Issue
7
Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement
© The Author(s) 2017. This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
Item Access Status
Note
Access the data
Related item(s)
Subject
Microbiology
Medical microbiology
Science & Technology
Life Sciences & Biomedicine
fucP
genotyping
Persistent link to this record
Citation
Price, EP; Harris, TM; Spargo, JC; Nosworthy, E; Beissbarth, J; Chang, AB; Smith-Vaughan, HC; Sarovich, DS, Simultaneous identification of Haemophilus influenzae and Haemophilus haemolyticus using real-time PCR, Future Microbiology, 2017, 12 (7), pp. 585-593