A Novel Thermostable Dextranase from a Thermoanaerobacter Species Cultured from the Geothermal Waters of the Great Artesian Basin of Australia
File version
Author(s)
Patel, BK
Bain, P
de Jersey, J
Hamilton, S
Inkerman, PA
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
Size
File type(s)
Location
License
Abstract
A Gram-negative sporulating thermophilic anaerobe, designated AB11Ad, was isolated from the heated waters of the Great Artesian Basin of Australia. It grew on a variety of carbohydrates including glucose, starch, and dextran and produced a thermostable and thermoactive extracellular endo-dextranase. The enzyme was produced more actively under pH controlled continuous culture conditions than under batch conditions. Ammonium sulfate precipitated crude dextranase exhibited a temperature optimum of 70 °C and a pH optimum between 5 and 6. The half life was ~ 6.5 h at 75 °C and 2 h at 80 °C at pH 5.0 and in the absence of added dextran. 16S rRNA sequence analysis indicated that isolate AB1 lAd was a member of the genus Thermoanaerobacter.
Journal Title
FEMS Microbiology Letters
Conference Title
Book Title
Edition
Volume
140
Issue
2-Mar
Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement
Item Access Status
Note
Access the data
Related item(s)
Subject
Biological sciences
Agricultural, veterinary and food sciences
Applications in life sciences
Biomedical and clinical sciences