A Novel Thermostable Dextranase from a Thermoanaerobacter Species Cultured from the Geothermal Waters of the Great Artesian Basin of Australia

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Wynter, C
Patel, BK
Bain, P
de Jersey, J
Hamilton, S
Inkerman, PA
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1996
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Abstract

A Gram-negative sporulating thermophilic anaerobe, designated AB11Ad, was isolated from the heated waters of the Great Artesian Basin of Australia. It grew on a variety of carbohydrates including glucose, starch, and dextran and produced a thermostable and thermoactive extracellular endo-dextranase. The enzyme was produced more actively under pH controlled continuous culture conditions than under batch conditions. Ammonium sulfate precipitated crude dextranase exhibited a temperature optimum of 70 °C and a pH optimum between 5 and 6. The half life was ~ 6.5 h at 75 °C and 2 h at 80 °C at pH 5.0 and in the absence of added dextran. 16S rRNA sequence analysis indicated that isolate AB1 lAd was a member of the genus Thermoanaerobacter.

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FEMS Microbiology Letters

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140

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2-Mar

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Biological sciences

Agricultural, veterinary and food sciences

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Biomedical and clinical sciences

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