Glycomic analysis of gastric carcinoma cells discloses glycans as modulators of RON receptor tyrosine kinase activation in cancer

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Author(s)
Mereiter, Stefan
Magalhaes, Ana
Adamczyk, Barbara
Jin, Chunsheng
Almeida, Andreia
Drici, Lylia
Ibanez-Vea, Maria
Gomes, Catarina
Ferreira, Jose A
Afonso, Luis P
Santos, Lucio L
Larsen, Martin R
Kolarich, Daniel
Karlsson, Niclas G
Reis, Celso A
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2016
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Abstract

Background:

Terminal α2-3 and α2-6 sialylation of glycans precludes further chain elongation, leading to the biosynthesis of cancer relevant epitopes such as sialyl-Lewis X (SLeX). SLeX overexpression is associated with tumor aggressive phenotype and patients' poor prognosis.

Methods:

MKN45 gastric carcinoma cells transfected with the sialyltransferase ST3GAL4 were established as a model overexpressing sialylated terminal glycans. We have evaluated at the structural level the glycome and the sialoproteome of this gastric cancer cell line applying liquid chromatography and mass spectrometry. We further validated an identified target expression by proximity ligation assay in gastric tumors.

Results:

Our results showed that ST3GAL4 overexpression leads to several glycosylation alterations, including reduced O-glycan extension and decreased bisected and increased branched N-glycans. A shift from α2-6 towards α2-3 linked sialylated N-glycans was also observed. Sialoproteomic analysis further identified 47 proteins with significantly increased sialylated N-glycans. These included integrins, insulin receptor, carcinoembryonic antigens and RON receptor tyrosine kinase, which are proteins known to be key players in malignancy. Further analysis of RON confirmed its modification with SLeX and the concomitant activation. SLeX and RON co-expression was validated in gastric tumors.

Conclusion:

The overexpression of ST3GAL4 interferes with the overall glycophenotype of cancer cells affecting a multitude of key proteins involved in malignancy. Aberrant glycosylation of the RON receptor was shown as an alternative mechanism of oncogenic activation.

General significance:

This study provides novel targets and points to an integrative tumor glycomic/proteomic-profiling for gastric cancer patients' stratification. This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc.

Abbreviations: SLea, Sialyl Lewis A; SLeX, Sialyl Lewis X; PTM, Post-translational modifications; RTK, Receptor tyrosine kinase; IHC, Immunohistochemistry; PLA, Proximity ligation assay; GC, gastric cancer; HILIC-FLD-UPLC, hydrophilic interaction liquid chromatography fluorescence detection ultra-performance liquid chromatography

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Biochimica et Biophysica Acta. General Subjects

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1860

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8

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Biochemistry and cell biology

Biochemistry and cell biology not elsewhere classified

Pharmacology and pharmaceutical sciences

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