We present new algorithms for segmenting neuron im- ages which are taken from cells being grown in culture with oxidative agents. Information from changing im- ages can be used to compare changes in neurons from the Zellweger mice to those from normal mice. Im- age segmentation is the first and major step for the study of these different types of processes in neuron cells. It is difficult to do it as these neuron cell im- ages from stained fields and unimodal histograms. In this paper we develop an innovative strategy for the segmentation of neuronal cell images which are sub- jected to stains and whose histograms are unimodal. The proposed method is based on logical analysis of grey difference. Two key parameters, window width and logical threshold, are automatically extracted to be used in logical thresholding method. Spurious re- gions are detected and removed by using hierarchical filtering window. Experiment and comparison results show the efficient of our algorithms.