Bioaffinity Mass Spectrometry Screening
File version
Author(s)
Feng, Yun Jiang
Vu, Hoan
McCormick, Brendan
Rowley, Jessica
Pedro, Liliana
Crowther, Gregory J
Van Voorhis, Wesley C
Forster, Paul I
Quinn, Ronald J
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
Size
File type(s)
Location
License
Abstract
Electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FTICR-MS or ESI-FTMS) was used to screen 192 natural product extracts and a 659-member natural product-based fragment library for bindings to a potential malaria drug target, Plasmodium falciparum Rab11a (PfRab11a, PF13_0119). One natural product extract and 11 fragments showed binding activity. A new natural product, arborside E, was identified from the active extract of Psydrax montigena as a weak binder. Its binding activity and inhibitory activity against PfRab11a were confirmed by ESI-FTMS titration experiments and an orthogonal enzyme assay.
Keywords
Journal Title
Journal of Biomolecular Screening
Conference Title
Book Title
Edition
Volume
21
Issue
2
Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement
Item Access Status
Note
Access the data
Related item(s)
Subject
Biochemistry and cell biology
Biochemistry and cell biology not elsewhere classified
Medical biochemistry and metabolomics
Medical biotechnology