Herbicides are chemical compounds that are toxic to weed plants. Modern agriculture relies heavily on herbicides for the control of weeds to maximize crop yields. Herbicide usage in the Australian grains industry is estimated to have increased by more than 65% from 2014 to 2024, which equates to more than AUD 2.50 billion dollars per year. The increased popularity of herbicides in farming systems has raised concerns about their negative impacts on the environment, human health and agricultural sustainability due to the rapid evolution of herbicide resistance, as well as their behaviour and fate in the soil. Due to excessive use of herbicides, soil and water pollution, reduced biodiversity and depression in soil heterotrophic bacteria (including denitrifying bacteria) and fungi are becoming increasingly common. Biological degradation governed by microorganisms serves as a major natural remediation process for a variety of pollutants including herbicides. This review provides a brief overview of the present status of herbicide residues in Australian farming systems, with a focus on the microbial degradation of herbicides in soil. It highlights key bacterial and fungal strains involved and the environmental factors influencing the biodegradation process. Recent advancements, including the application of omics technologies, are outlined to provide a comprehensive understanding of the biodegradation process.