Neutrophils are currently connected with a more ominous role in cancer progression. The problem is that neutrophils are made up of a diverse group of immune cells, and many of the cancer studies have attempted to group them together without taking into account the distinctions in subgroups that exist within the neutrophil population. The Ralph laboratory, utilizing mouse models of breast cancer, has revealed that the neutrophil subtype N1 (anticancer as opposed to the N2 pro-tumour) may be directed for recruitment to the tumour site by employing suitable anticancer drugs as effective pro-oxidants given intra-tumourally (22). In a similar manner, the studies outlined here were aimed at establishing whether injecting the pro-oxidant, hydrogen peroxide (H2O2) or the combination of H2O2 and celecoxib (CXB) treatment into breast cancers, would promote neutrophils as effective cytotoxic anticancer agents significantly inhibiting tumour development to the stage of remission as was shown previously using the Tea Tree Oil derived pro-oxidants (22). Hence, the goal of the current project was to build on the prior research conducted in the Ralph laboratory but to examine whether there was an immune cell-based mechanism relating to the Kochi oxydol radiation therapy for unresectable carcinomas II (KORTUC II) approach. These studies utilised the transgenic FVB/N c-neu mice to examine the therapeutic effectiveness of H2O2, a pro-oxidant, against cancer. [...]