INTRODUCTION: Our aim is to optimise the residuum health of individuals suffering from lower-limb loss through computational simulations of a 'Digital Twin'; that will facilitate the design of optimized 3D-printed prosthetic attachments. Dynamic Anatomical Ultrasonography (DAU) will provide a primary input into the computational simulations, facilitating 4D-tracking of skin/fat, fat/muscle, and muscle/bone tissue interfaces throughout a residuum during ambulation. METHOD: With reference to Figure 1, a DAU Workflow has been created to facilitate the development of novel instrumentation and analyses. For DAU analysis, A-scan signals are recorded over a defined time from a series of ultrasound transducers. The proprietary format datafile is interrogated utilizing a Matlab program to provide plots of various tissueinterface depths verses recording time. Proof-of Concept analysis has been performed on a replica skinfat-muscle tissue test sample, shown in Figure 2. CONCLUSIONS: A DAU Workflow has been developed. This is being implemented utilising a CT scan of an asymptomatic subject fitted with a pseudo-socket; with image analysis, CAD and 3D-printing facilitating creation of a bespoke device for DAU assessment in-vivo.