Aim The primary aim of this study was to evaluate the impact of a targeted, practical education intervention on paediatric nurses' knowledge of appropriate breastfeeding management in an acute practice setting. Background Breastfeeding provides the optimal method for infant growth and nutrition, yet studies have demonstrated knowledge deficits of Australian paediatric nurses regarding the management of the mother-infant breastfeeding dyad. Limited evidence is available evaluating which educational approach is effective in the dynamic and busy acute care setting. Design This study was a two-phased, mixed-method design, conducted in a large, tertiary, metropolitan children's hospital in south-east Queensland, Australia. Methods Reference groups were conducted in Phase One to advise development of the resource kit. Phase Two was the experimental phase and included a pre-test knowledge survey, the educational intervention, and a post-test knowledge survey. Results Pre- and post-test response rates of 75% (n=49) and 34% (n=23) respectively were achieved from the population of 67 eligible participants. Post-intervention study results demonstrated knowledge improvement in four key breastfeeding management areas: importance of baby-led feeding; reduction in otitis media risk for breastfed infants; ongoing management of maternal milk supply when breastfeeding is interrupted; and the correct storage and management of expressed breast milk. Areas for further knowledge improvement included management of more complex breastfeeding scenarios, such as mastitis. Conclusion The implementation of a resource kit and brief education series has improved the knowledge of paediatric nurses in some areas of breastfeeding practice and management.