Precision bioengineering of the well-known production host E.coli to synthesize self-assembled polymers has led to many novel applications. This thesis aims to explore vaccine and diagnostic tool development for Q fever using bacterial polymers synthesized with PHB technology developed in the Rehm lab. In addition, we also explored an immunogenic protein carrier, CRM197, as an alternative platform for Q fever vaccine development. Coxiella burnetii, the etiological agent of Q-fever, is considered a risk group 3 pathogen owing to its highly infectious nature. The primary form of prevention is through vaccination, and the current vaccine, Q-VAX, induces adverse reactions that hinder its worldwide use. This necessitates a safe alternative vaccine, and we provide evidence for a cost-effective, ambient temperature stable biopolymer and CRM197 particle-based subunit vaccine that induces protection against C. burnetii. To this end, we also developed a diagnostic tool that replaces the need for infectious C. burnetii as a diagnostic marker and functions as a potential rapid test for the Q fever vaccination pre-screening process.