Nanostructured Graphene Surfaces Promote Different Stages of Bone Cell Differentiation

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Borghi, FF
Bean, PA
Evans, MDM
van der Laan, T
Kumar, S
Ostrikov, K
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2018
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Abstract

Nanostructured graphene films were used as platforms for the differentiation of Saos-2 cells into bone-like cells. The films were grown using the plasma-enhanced chemical vapor deposition method, which allowed the production of both vertically and horizontally aligned carbon nanowalls (CNWs). Modifications of the technique allowed control of the density of the CNWs and their orientation after the transfer process. The influence of two different topographies on cell attachment, proliferation, and differentiation was investigated. First, the transferred graphene surfaces were shown to be noncytotoxic and were able to support cell adhesion and growth for over 7 days. Second, early cell differentiation (identified by cellular alkaline phosphatase release) was found to be enhanced on the horizontally aligned CNW surfaces, whereas mineralization (identified by cellular calcium production), a later stage of bone cell differentiation, was stimulated by the presence of the vertical CNWs on the surfaces. These results show that the graphene coatings, grown using the presented method, are biocompatible. And their topographies have an impact on cell behavior, which can be useful in tissue engineering applications.[Figure not available: see fulltext.].

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Nano-Micro Letters

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10

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3

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© The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Nanomaterials

Science & Technology

Technology

Physical Sciences

Nanoscience & Nanotechnology

Materials Science, Multidisciplinary

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Borghi, FF; Bean, PA; Evans, MDM; van der Laan, T; Kumar, S; Ostrikov, K, Nanostructured Graphene Surfaces Promote Different Stages of Bone Cell Differentiation, Nano-Micro Letters, 2018, 10 (3), pp. 47:1-47:12

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