Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: a retrospective re-analysis.
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Binks, MJ
Marsh, RL
Chang, AB
Leach, AJ
Smith-Vaughan, HC
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Abstract
Objective: Haemophilus haemolyticus can be misidentified as nontypeable Haemophilus influenzae (NTHi) due to their phenotypic similarities in microbiological culture. This study aimed to determine the prevalence of misidentified NTHi in respiratory specimens from children living in northern Australia.
Results: Among respiratory specimens collected in studies between 2010 and 2013, retrospective PCR analysis found that routine culture misidentified H. haemolyticus as NTHi in 0.3% (3/879) of nasal specimens, 25% (14/55) of bronchoalveolar lavage and 40% (12/30) of throat specimens. Therefore, in this population, PCR-based NTHi diagnostics are indicated for throat and bronchoalveolar specimens, but not for nasal specimens.
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BMC Research Notes
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11
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1
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© The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
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Biochemistry and cell biology
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Haemophilus haemolyticus (Hh)
Nasopharynx, bronchoalveolar lavage
Nontypeable Haemophilus influenzae (NTHi)
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Beissbarth, J; Binks, MJ; Marsh, RL; Chang, AB; Leach, AJ; Smith-Vaughan, HC, Recommendations for application of Haemophilus influenzae PCR diagnostics to respiratory specimens for children living in northern Australia: a retrospective re-analysis., BMC Research Notes, 2018, 11 (1), pp. 323-