Performance of AmpFire HPV assay on neck cervical lymph node aspirate and oropharyngeal samples

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Author(s)
Jang, Dan
Shah, Anika
Arias, Manuel
Ratnam, Sam
Smieja, Marek
Chen, Xin
Wang, Youxiang
Speicher, David J
Chernesky, Max
Griffith University Author(s)
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2020
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Abstract

Early determination of high-risk human papillomaviruses causing oropharyngeal squamous cell carcinomas (OPSCC) may influence treatment. The objectives were to evaluate the performance of a new rapid isothermal nucleic acid amplification point of care HPV test (AmpFire HPV) on fine needle neck aspirates (FNA) of cervical lymph nodes and oropharyngeal swabs and saliva (OPS) which had been previously tested by the cobas HPV assay. The comparison was performed on 56 FNA and 81 OPS. The two assays showed strong agreement (94.6 %, K = 0.88) on FNA and fair agreement (65.4 %, K = 0.34) on OPS. AmpFire HPV performed on FNA demonstrated a sensitivity of 76.7 % and specificity of 81.8 % for the prediction of p16 antigens in OPSCC with results available in 1.5 h.

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Journal of Virological Methods

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279

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Microbiology

Medical microbiology

Science & Technology

Life Sciences & Biomedicine

Biochemical Research Methods

Biotechnology & Applied Microbiology

Virology

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Jang, D; Shah, A; Arias, M; Ratnam, S; Smieja, M; Chen, X; Wang, Y; Speicher, DJ; Chernesky, M, Performance of AmpFire HPV assay on neck cervical lymph node aspirate and oropharyngeal samples, Journal of Virological Methods, 2020, 279

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