The aim of this paper is to describe the practical application of a reflective knowledge development lifecycle (KDLC) designed to be used by organisations reviewing their KM processes and capability. The KDLC had emerged from practical experience but had not been more widely validated. The present study seeks to provide a unique opportunity to test theory comprehensively against an exemplary case where both organisation and investigator were suited to the work. Design/methodology/approach - A case study research strategy was used. Applying the theory in a practical setting, an environmental scientist used the model and associated knowledge representation and visualisation tools to find gaps in processes and design in the implementation of an organisation-wide research framework. Findings - Developing the knowledge repository highlighted inherent complexity among people, resources, research activities, operational tasks and communication outcomes. The study helped validate the KDLC's utility in ensuring alignment of organisational processes and strategy; demonstrating its useful application in a practical setting. Research limitations/implications - A replication across several organisations would help to refine the model beyond this critical test in a single organisation, while suggesting further practical recommendations. Originality/value - The results of the study suggest that the KDLC is of value to practitioners in providing a check-list approach for knowledge auditing, to theorists developing organisational KM models and those using test-case strategies. The outcomes will particularly interest information and environmental professionals involved in establishing adaptive management projects.