A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma
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Day, Bryan W
D'Souza, Rochelle CJ
Jamieson, Paul R
Ensbey, Kathleen S
Bruce, Zara C
Lim, Yi Chieh
Goasdoue, Kate
Offenhauser, Carolin
Akgul, Seckin
Allan, Suzanne
Robertson, Thomas
Lucas, Peter
Tollesson, Gert
Campbell, Scott
et al.
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Abstract
Low-passage, serum-free cell lines cultured from patient tumour tissue are the gold-standard for preclinical studies and cellular investigations of glioblastoma (GBM) biology, yet entrenched, poorly-representative cell line models are still widely used, compromising the significance of much GBM research. We submit that greater adoption of these critical resources will be promoted by the provision of a suitably-sized, meaningfully-described reference collection along with appropriate tools for working with them. Consequently, we present a curated panel of 12 readily-usable, genetically-diverse, tumourigenic, patient-derived, low-passage, serum-free cell lines representing the spectrum of molecular subtypes of IDH-wildtype GBM along with their detailed phenotypic characterisation plus a bespoke set of lentiviral plasmids for bioluminescent/fluorescent labelling, gene expression and CRISPR/Cas9-mediated gene inactivation. The cell lines and all accompanying data are readily-accessible via a single website, Q-Cell (qimrberghofer.edu.au/q-cell/) and all plasmids are available from Addgene. These resources should prove valuable to investigators seeking readily-usable, well-characterised, clinically-relevant, gold-standard models of GBM.
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Scientific Reports
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9
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© The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
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Oncology and carcinogenesis
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
STEM-CELLS
HETEROGENEITY
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Stringer, BW; Day, BW; D'Souza, RCJ; Jamieson, PR; Ensbey, KS; Bruce, ZC; Lim, YC; Goasdoue, K; Offenhauser, C; Akgul, S; Allan, S; Robertson, T; Lucas, P; Tollesson, G; Campbell, S; Winter, C; Do, H; Dobrovic, A; Inglis, P-L; Jeffree, RL; Johns, TG; Boyd, A, A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma, Scientific Reports, 2019, 9, pp. 4902