Good hand hygiene and “scrub the hub” practices are important to prevent bloodstream infections. This observational study (n = 108) found high compliance with “scrubbing the hub,” although scrub time was shorter than the recommended duration (average 6.1 seconds). Compliance with hand hygiene before medication preparation (33%) and before medication administration (43%) showed room for improvement compared with postadministration (65%), the emergency setting and glove use were associated with poorer compliance (P <.01).