For thermostated dissipative systems, the fluctuation theorem gives an analytical expression for the ratio of probabilities that the time-averaged entropy production in a finite system observed for a finite time takes on a specified value compared to the negative of that value. In the past, it has been generally thought that the presence of some thermostating mechanism was an essential component of any system that satisfies a fluctuation theorem. In the present paper, we point out that a fluctuation theorem can be derived for purely Hamiltonian systems, with or without applied dissipative fields.