It is necessary to find effective methods for quantifying ecosystem stability that can forewarn massive collapses of ecosystem structure and inform ecosystem management. Multiple ecosystem stability metrics (ESMs) have been proposed and are increasingly employed to quantify terrestrial ecosystem stability. However, their sensitivity and consistency in depicting stability changes in diverse terrestrial ecosystems have not been thoroughly examined. Here, we evaluated 11 commonly suggested ESMs using two vegetation indices (normalized difference vegetation index, NDVI, and enhanced vegetation index, EVI) and examined their responses to the climatic gradient in diverse African biomes. We found that ESMs exhibited unequal sensitivity as certain metrics showed insignificant trends with climatic drivers whereas others indicated significant changes in stability in the same case. Among the significant results, inconsistent trends indicated by different ESMs were prevalent. Moreover, diverse ESMs exhibited mostly weak correlations despite being based on the same theoretical deduction. The ESMs derived from NDVI and EVI exhibited inconsistencies in some cases, suggesting the influence of the adopted variables on ESM performance. Our study revealed that multiple ESMs were unequally sensitive and inconsistent in indicating ecosystem stability. ESM performance was synthetically influenced by the ESM algorithm (different metrics), the adopted variable for ESM calculation, and the target biome type. Improvements in ecosystem stability evaluation on broad scales, therefore, are needed through further exploration and verification of ESMs.