Microbial metabolism of thiopurines: A method to measure thioguanine nucleotides
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Author(s)
Movva, Ramya
Lobb, Michael
Cuiv, Paraic O
Florin, Timothy Fij
Duley, John A
Oancea, Iulia
Lobb, Michael
Cuiv, Paraic O
Florin, Timothy Fij
Duley, John A
Oancea, Iulia
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2016
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Abstract
Thiopurines are anti-inflammatory prodrugs. We hypothesised that bacteria may contribute to conversion to active drug. Escherichia coli strain DH5α was evaluated to determine whether it could metabolise the thiopurine drugs, thioguanine or mercaptopurine, to thioguanine nucleotides. A rapid and reliable high performance liquid chromatography (ultraviolet detection) method was developed to quantify indirectly thioguanine nucleotides, by measuring thioguanine nucleoside.
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Journal of Microbiological Methods
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128
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Microbiology
Microbiology not elsewhere classified
Medical microbiology