The genus Spiraea L., spirea, represents deciduous shrubs of the family Rosaceae Juss., subfamily Spiraeoideae Focke. The genus is widespread in the temperate and the subtropical zone of the northern hemisphere having more than 100 species. S. hypericifolia L. has the most extensive Eurasian range and is considered one of the most evolutionarily advanced representatives of the genus. In the leaves of S. hypericifolia L. detected, phydroxybenzoic, coffee, ferulic, chlorogenic acid, flavones apigenin, luteolin and 5-glucosides of flavonols isoquercitrin and avicularin. In this study, chemical constituents of liposoluble extract of Spiraea hypericifolia L. were determined for the first time. The components isolated from liposoluble extract of the aerial part of medicinal plant of S. hypericifolia L. were analyzed using the GC-MS method. In total, sixty-three compounds were isolated from hexane part and their relative content was determined by normalizing the peak area, in which the main components are octacosanol (9.59%), docosene-1 (8.57%), squalene (8.12%), and n-hexadecanoic acid (7.50%) separately. These compounds have high biological activites, namely anti-inflammatory, antimicrobial, antitumor, antibacterial.