Single-band upconversion nanoprobes for multiplexed simultaneous in situ molecular mapping of cancer biomarkers

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Zhou, Lei
Wang, Rui
Yao, Chi
Li, Xiaomin
Wang, Chengli
Zhang, Xiaoyan
Xu, Congjian
Zeng, Aijun
Zhao, Dongyuan
Zhang, Fan
Griffith University Author(s)
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2015
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Abstract

The identification of potential diagnostic markers and target molecules among the plethora of tumour oncoproteins for cancer diagnosis requires facile technology that is capable of quantitatively analysing multiple biomarkers in tumour cells and tissues. Diagnostic and prognostic classifications of human tumours are currently based on the western blotting and single-colour immunohistochemical methods that are not suitable for multiplexed detection. Herein, we report a general and novel method to prepare single-band upconversion nanoparticles with different colours. The expression levels of three biomarkers in breast cancer cells were determined using single-band upconversion nanoparticles, western blotting and immunohistochemical technologies with excellent correlation. Significantly, the application of antibody-conjugated single-band upconversion nanoparticle molecular profiling technology can achieve the multiplexed simultaneous in situ biodetection of biomarkers in breast cancer cells and tissue specimens and produce more accurate results for the simultaneous quantification of proteins present at low levels compared with classical immunohistochemical technology.

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Nature Communications

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6

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1

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© The Author(s) 2015. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Science & Technology

Multidisciplinary Sciences

Science & Technology - Other Topics

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Zhou, L; Wang, R; Yao, C; Li, X; Wang, C; Zhang, X; Xu, C; Zeng, A; Zhao, D; Zhang, F, Single-band upconversion nanoprobes for multiplexed simultaneous in situ molecular mapping of cancer biomarkers, Nature Communications, 2015, 6 (1)

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