Improved genetic markers for monitoring recruitment dynamics in the endangered Mary River cod (Maccullochella mariensis)

Loading...
Thumbnail Image
File version

Accepted Manuscript (AM)

Author(s)
Schmidt, DJ
Espinoza, T
Real, K
Dunlop, A
Kennard, M
Hughes, JM
Primary Supervisor
Other Supervisors
Editor(s)
Date
2018
Size
File type(s)
Location
License
Abstract

The Mary River cod (Maccullochella mariensis) is a large predatory freshwater fish identified as a potential flagship for freshwater ecosystem conservation in Australia. The species is endemic to the Mary River catchment in eastern Australia, and is listed as Endangered. Previous conservation genetic assessment of Mary River cod was based on a small set of microsatellite loci developed for congeneric Maccullochella species. Here we develop a novel set of 15 microsatellite loci specific to M. mariensis, and demonstrate that these markers exhibit higher variability than those used previously. Mean number of alleles per locus was 4 and mean expected heterozygosity was 0.57. We genotyped 35 Mary River cod larvae belonging to a single cohort using the 15 novel loci and eight previously used loci, and found 10 full‐sib family groups along with clear genetic differentiation between individuals collected from the two sub‐catchments – Tinana Creek and Mary River. Microsatellites presented here will be useful for cost‐effective monitoring of genetic diversity and recruitment dynamics in this endangered fish species.

Journal Title

Journal of Applied Ichthyology

Conference Title
Book Title
Edition
Volume

34

Issue

3

Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement

© 2018 Journal of Applied Ichthyology. This is the peer reviewed version of the following article: Improved genetic markers for monitoring recruitment dynamics in the endangered Mary River cod (Maccullochella mariensis), Journal of Applied Ichthyology, Volume 34, Issue 3, Pages 633-637, 2018, which has been published in final form at https://doi.org/10.1111/jai.13633. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving (http://olabout.wiley.com/WileyCDA/Section/id-828039.html)

Item Access Status
Note
Access the data
Related item(s)
Subject

Zoology

Zoology not elsewhere classified

Other biological sciences

Fisheries sciences

Persistent link to this record
Citation
Collections