Assessment of yellowtail kingfish (Seriola lalandi) as a surrogate host for the production of southern bluefin tuna (Thunnus maccoyii) seed via spermatogonial germ cell transplantation

No Thumbnail Available
File version
Author(s)
Bar, I
Smith, A
Bubner, E
Yoshizaki, G
Takeuchi, Y
Yazawa, R
Chen, BN
Cummins, S
Elizur, A
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
2016
Size
File type(s)
Location
License
Abstract

Germ cell transplantation is an innovative technology for the production of interspecies surrogates, capable of facilitating easier and more economical management of large-bodied broodstock, such as the bluefin tuna. The present study explored the suitability of yellowtail kingfish (Seriola lalandi) as a surrogate host for transplanted southern bluefin tuna (Thunnus maccoyii) spermatogonial cells to produce tuna donor-derived gametes upon sexual maturity. Germ cell populations in testes of donor T. maccoyii males were described using basic histology and the molecular markers vasa and dead-end genes. The peripheral area of the testis was found to contain the highest proportions of dead-end-expressing transplantable Type A spermatogonia. T. maccoyii Type A spermatogonia-enriched preparations were transplanted into the coelomic cavity of 6–10-day-old post-hatch S. lalandi larvae. Fluorescence microscopy and polymerase chain reaction analysis detected the presence of tuna cells in the gonads of the transplanted kingfish fingerlings at 18, 28, 39 and 75 days after transplantation, indicating that the transplanted cells migrated to the genital ridge and had colonised the developing gonad. T. maccoyii germ cell-derived DNA or RNA was not detected at later stages, suggesting that the donor cells were not maintained in the hosts’ gonads.

Journal Title

Reproduction, Fertility and Development

Conference Title
Book Title
Edition
Volume

28

Issue

12

Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement
Item Access Status
Note
Access the data
Related item(s)
Subject

Biological sciences

Agricultural, veterinary and food sciences

Biomedical and clinical sciences

Animal production

Zoology

Reproductive medicine

Science & Technology

Life Sciences & Biomedicine

Developmental Biology

Reproductive Biology

Zoology

Persistent link to this record
Citation

Bar, I; Smith, A; Bubner, E; Yoshizaki, G; Takeuchi, Y; Yazawa, R; Chen, BN; Cummins, S; Elizur, A, Assessment of yellowtail kingfish (Seriola lalandi) as a surrogate host for the production of southern bluefin tuna (Thunnus maccoyii) seed via spermatogonial germ cell transplantation, Reproduction, Fertility and Development, 2016, 28 (12), pp. 2051-2064

Collections