Tracking the Source of Escherichia coli isolated from Somerset, Baroon Pocket and Wivenhoe dams Using the B-glucuronidase gene : data
File version
Author(s)
Schroeder, Sarah
Christie, Melody
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
Size
File type(s)
.doc
.xls
.tiff
other
Location
Wivenhoe Dam;27°23′38″S 152°36′28″E; Somerset Dam 27.1144° S, 152.5570° E; Baroon Pocket Dam 26.7032° S, 152.8680° E
License
Abstract
The collection contains a lab notebook, as well as associated spreadsheets, documents, images, and phylogenetic analysis files. The data was collected as part of a UWSRA funded project investigating the reduction of selected biological and chemical contaminants in South East Queensland reservoirs and associated waterways. The data is part of an ongoing method development research program funded by the Water Quality Research Centre with Seqwater as industry partners. E.coli isolates were collected by Seqwater from Wivenhoe Dam, Somerset Dam and Baroon Pocket Dam, and provided as live cultures to the research team at the Smart Water Research Centre. The isolates were checked for purity and stored in nutrient broth & 30% glycerol at -80oC until analysed. A 518 base pair fragment of the beta-glucuronidase gene from all E. coli isolates was amplified, using the polymerase chain reaction and sequenced. The sequence data from unknown isolates was compared to a library of known E. coli beta-glucuronidase gene sequences. Multiple sequence alignments were performed using ClustalX. Gap-only columns in the multiple sequence alignment were removed and final editing of the alignment was performed in BioEdit to yield an aligned 518 base pair fragment. Phylogenetic analysis involved the construction of phylogentic trees using the Neighbour-Joining method. The NJ tree was generated using MEGA version 5 employing the Maximum Composite Likelihood model/method and 1,000 bootstrap replicates.
Journal Title
Conference Title
Book Title
Edition
Volume
Issue
Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement
Copyright is held by the creator, unless otherwise stated.
Item Access Status
Mediated access. Data may be made available for collaborative research purposes. Contact the data custodian (Helen Stratton) to discuss access to this data collection. Access requests via the mediated request form link.
Note
Access the data
Related item(s)
Subject
Bacteriology
Microbial Ecology
Microbial Genetics
water quality
water contaminants
pathogens
bacteria
reservoirs
dams
management
microbial Source Tracking
E.coli
beta-glucuronidase gene
catchment management