Inhibition of Caco-2 and HeLa proliferation by Terminalia sericea Burch. ex DC. (Combretaceae) leaf extracts and identification of volatile components

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Cock, IE
Gu, B
Sirdaarta, J
Van Vuuren, SF
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2017
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Abstract

Terminalia sericea Burch. ex. DC (Combretaceae) is used in traditional South African medicine to treat a wide variety of diseases. Several other Terminalia spp. have been reported to have anti-proliferative and apoptotic activities. Despite this, the anticancer properties of T. sericea remain to be rigorously tested. The current study was undertaken to test a panel of T. sericea leaf extracts for the ability to inhibit the proliferation of Caco-2 colorectal and HeLa cervical carcinoma cells, and to identify volatile compounds using GC-MS headspace analysis. Powdered T. sericea leaves were extracted with solvents of varying polarity and investigated for the ability to inhibit the proliferation of Caco-2 and cervical carcinoma cells using MTS based colorimetric cell proliferation assays. The extracts were tested for toxicity in the Artemia nauplii bioassay and the extract with the most potent anti-proliferative activity was examined using headspace GC-MS analysis to identify volatile nonpolar components. The T. sericea leaf extracts displayed potent anti-proliferative activity against Caco-2 and HeLa carcinoma cells. The methanolic extracts were particularly potent inhibitors of Caco-2 and HeLa proliferation. The aqueous and ethyl acetate extracts (but not the chloroform or hexane extracts) also significantly inhibited carcinoma proliferation, albeit with substantially lower potency. Subsequent analysis of the T. sericea leaf extracts by GC-MS headspace analysis highlighted several interesting volatile compounds. All extracts were shown to be non-toxic in the Artemia nauplii bioassay. The lack of toxicity of these extracts and their anti-proliferative bioactivity against Caco-2 and HeLa carcinoma cells indicates their potential in the treatment of some cancers.

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South African Journal of Botany

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109

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© The Author(s) 2017. This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Ecology

Plant biology

Science & Technology

Life Sciences & Biomedicine

Plant Sciences

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Cock, IE; Gu, B; Sirdaarta, J; Van Vuuren, SF, Inhibition of Caco-2 and HeLa proliferation by Terminalia sericea Burch. ex DC. (Combretaceae) leaf extracts and identification of volatile components, South African Journal of Botany, 2017, 109, pp. 329-330