Introduction: Closely related plant species often share similar secondary metabolites and bioactivities and are therefore good targets for bioactivity testing when one or more species within a genus are known to possess therapeutic properties. The genus Eucalyptus has a long history of medicinal usage by the first Australians. Many species are known to have therapeutic properties, several species of which have well established antibacterial bioactivities. Methods: The ability of Eucalyptus grandis leaf and flower extracts to inhibit the growth of a panel of bacterial and fungal pathogens was investigated by disc diffusion assay. Toxicity was examined using the Artemia franciscana nauplii bioassay. Results: E. grandis leaf and flower methanolic and aqueous extracts were completely ineffective at inhibiting the growth of gram-positive and gram-negative panels of bacteria, as well as fungi. The extracts were nontoxic in the Artemia nauplii bioassay following 24 hr exposure. Conclusion: Despite the taxonomic relationship to several bioactive Eucalyptus spp., E. grandis leaf and flower extracts were completely ineffective bacterial and fungal growth inhibitors. However, these extracts may have other therapeutic properties and testing against protozoa, virus and tumour cells is required.