Evaluation of shape and size changes of bone and remodelled bone substitute after different fixation methods

Loading...
Thumbnail Image
File version
Author(s)
Gedrange, T
Mai, R
Mack, F
Zietek, M
Borsos, G
Vegh, A
Spassov, A
Gredes, T
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
2008
Size

171007 bytes

File type(s)

application/pdf

Location
License
Abstract

Suitable tissue fixation is indispensable to histological analysis. This investigation, therefore, sought to evaluate changes of shape and size of bone specimens and remodelled bone substitute material following different fixation methods. Mandibular bones of 9 pigs (Sus scrofa domesticus) served as specimens. Two mandibular premolars were extracted respectively and the extraction alveoli were filled with synthetic bone substitute material. The samples were collected after 70 days. Fixation of 6 specimens respectively was done for 7 days in 4% formalin (formaldehyde), 70% ethanol and glycerol at 18àroom temperature. The samples were radiographically examined before and after fixation using a reference specimen and subsequently underwent histological analysis. After fixation in formalin, the samples showed no size changes. After fixation in glycerol, morphological analysis revealed minor shape changes. Fixation in ethanol causes shrinking of the tissue specimens. Histological inspection of the tissues shows no morphological changes except slight shrinking. In conclusion there is no universal fixative that could met all requirements and permited proper examination without affecting tissues or bone specimens.

Journal Title

Journal of Physiology and Pharmacology

Conference Title
Book Title
Edition
Volume

59

Issue

SUPPL 5

Thesis Type
Degree Program
School
DOI
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement

© 2008 the Journal of Physiology and Pharmacology and the Polish Physiological Society. The attached file is reproduced here in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitive, published version.

Item Access Status
Note
Access the data
Related item(s)
Subject

Dentistry not elsewhere classified

Pharmacology and pharmaceutical sciences

Medical physiology

Persistent link to this record
Citation
Collections