The use of strengthening techniques such as chemical grouting to inject chemical additives into the subsurface of soils to bind their particles together is often expensive, contain hazardous substances, and require heavy machinery for distribution. Microbial induced calcite precipitation (MICP) offers an alternative method to potentially improve the strength and stiffness of soil uniformity. This present study was aimed at isolating and characterising strains of urease producing bacteria capable of precipitating calcium carbonate, and to investigate urea hydrolysis of the selected strains compared with the conventionally used Sporosarcina pastuerii (DSM 33) strain. A total of twenty-eight bacterial strains were isolated from the selective enrichment culture medium. Designated strains LPB19, TSB31, TSB12, NB40, LPB4, LB5, and TSB29 were selected out of the twenty-eight bacterial strains based on their quick urease production. This study reports the isolation and characterisation of urease producing bacteria from Sarawak caves and their role in calcite precipitation. The bacterial strains isolated from the limestone caves possessed urease production abilities, and hydrolysed high concentration of urea. Further investigation should be carried out to optimise the physiochemical parameters necessary for urease production and determine the efficiency of the ureolytic strains on their ability to induce calcium carbonate.