Morphometric Changes of Osteocyte Lacunar in Diabetic Pig Mandibular Cancellous Bone

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Yao, Sheng
Du, Zhibin
Xiao, Lan
Yan, Fuhua
Ivanovski, Saso
Xiao, Yin
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2023
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Abstract

Osteocytes play an important role in bone metabolism. The interactions of osteocytes with the surrounding microenvironment can alter cellular and lacunar morphological changes. However, objective quantification of osteocyte lacunae is challenging due to their deep location in the bone matrix. This project established a novel method for the analytical study of osteocytes/lacunae, which was then used to evaluate the osteocyte morphological changes in diabetic pig mandibular bone. Eight miniature pigs were sourced, and diabetes was randomly induced in four animals using streptozotocin (STZ) administration. The mandibular tissues were collected and processed. The jawbone density was evaluated with micro-CT. Osteocyte lacunae were effectively acquired and identified using backscattered electron scanning microscopy (BSE). A significantly decreased osteocyte lacunae size was found in the diabetic group. Using the acid etching method, it was demonstrated that the area of osteocyte and lacunae, and the pericellular areas were both significantly reduced in the diabetes group. In conclusion, a standard and relatively reliable method for analyzing osteocyte/lacunae morphological changes under compromised conditions has been successfully established. This method demonstrates that diabetes can significantly decrease osteocyte/lacunae size in a pig’s mandibular cancellous bone.

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Biomolecules

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13

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1

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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Biochemistry and cell biology

Bioinformatics and computational biology

Medical biotechnology

Science & Technology

Life Sciences & Biomedicine

Biochemistry & Molecular Biology

osteocyte

bone

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Yao, S; Du, Z; Xiao, L; Yan, F; Ivanovski, S; Xiao, Y, Morphometric Changes of Osteocyte Lacunar in Diabetic Pig Mandibular Cancellous Bone, Biomolecules, 2023, 13 (1), pp. 49

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