Hydrocortisone and TGF-ß1 regulate the glucocorticoid receptor and the serotonin transporter in SWAN-71 and JEG-3 cells

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Rose'Meyer, Roselyn

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Nirthanan, Selvanayagam

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2019-02-27
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Introduction Pregnancy-related disorders such as pre-eclampsia (PE), intrauterine growth restriction (IUGR), and pre-term birth (PTB) significantly contribute to maternal and fetal morbidity and mortality in all human societies. Each of these conditions can result from placental insufficiency, a pathophysiological condition affecting the placenta due to trophoblast dysfunction. Maternal complications during pregnancy such as gestational diabetes mellitus (GDM) and perinatal depression (PD) show significant associations with these placental pathologies. Perturbations in stress and inflammatory mediators, cortisol and TGF-β1, as well as the serotonin transporter of the serotonin system, have been associated with abnormal properties of trophoblast function including proliferation, migration, invasion, and remodelling which underpin trophoblast dysfunction and incomplete placentation. While glucocorticoid signalling, TGF-β1 signal transduction, and the serotonin system have been implicated in the aetiologies of these pathological conditions, their interactions have been seldom explored in placental cell models. Experimental aims The aim of the present study was to investigate the effects of stress and inflammatory mediators, cortisol and TGF-β1, on cell metabolic activity and cytotoxicity measured using the MTT and LDH assays, protein expression of glucocorticoid receptor isoforms and the serotonin transporter measured using Western blot analysis, as well as mRNA expression of the glucocorticoid receptor measured using NanoString nCounter analysis in placental cell lines, SWAN-71 and JEG-3. The purpose of these experiments was to elucidate the relationships between stress, inflammation, and the serotonin system in trophoblast pathophysiology and possible involvement in the aetiologies of PE, IUGR, and PTB. [...]

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Thesis (Masters)

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Master of Medical Research (MMedRes)

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School of Medical Science

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The author owns the copyright in this thesis, unless stated otherwise.

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Subject

Pregnancy-related disorders

Placental insufficiency

Maternal complications

Stress and inflammatory mediators

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