A pilot study to investigate the feasibility of transporting saliva samples at room temperature with MAWI Cell Stabilization buffer

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Lim, Yenkai
Punyadeera, Chamindie
Griffith University Author(s)
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Saliva is considered as the front-line of non-invasive diagnostics as novel biomarkers continue to emerge for an array of systemic diseases. Biomarker development pipeline relies heavily on pre-analytical process such as saliva collection, handling, transport and storage. The aim of this study was to systematically evaluate the applicability of MAWI Cell Stabilization (MCS) buffer to transport and store saliva samples at room temperature for downstream applications. Human and bacterial genomic DNA (gDNA) and total protein in saliva samples with and without MCS buffer were quantified for a week at three time-points at room temperature. Based on our findings, MCS buffer was able to preserve human gDNA and total protein within the testing time-points. While bacterial gDNA was accurately preserved, MCS buffer was unable to halt bacterial growth at room temperature. We have identified a non-alcohol-based, non-lytic buffer that could maintain the integrity of both genomic materials and proteins in saliva samples. MCS buffer offers a method to potentially transport and store saliva samples at room temperature, accelerating the translation of salivary assays in remote/rural and resource limited settings.

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Cogent Biology

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© 2018 The Author(s). This open access article is distributed under a Creative Commons Attribution (CC-BY) 4.0 license You are free to: Share — copy and redistribute the material in any medium or format. Adapt — remix, transform, and build upon the material for any purpose, even commercially. The licensor cannot revoke these freedoms as long as you follow the license terms. Under the following terms: Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. No additional restrictions You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

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Lim, Y; Punyadeera, C, A pilot study to investigate the feasibility of transporting saliva samples at room temperature with MAWI Cell Stabilization buffer, Cogent Biology 2018, 4 (1), pp. 1470895