Midpregnancy testing for soluble fms-like tyrosine kinase 1 (sFlt-1) and placental growth factor (PlGF): An inter-assay comparison of three automated immunoassay platforms
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Al-Amin, Ahmed
Rolnik, Daniel Lorber
Kane, Stefan C
Stolarek, Caroline
White, Adrienne
Costa, Fabricio da Silva
Brennecke, Shaun
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Abstract
We performed an inter-assay comparison among three immunoassay platforms for midpregnancy testing of sFlt-1, PlGF and the sFlt-1/PlGF ratio, which are established markers for pre-eclampsia. Maternal blood was collected 19–22 weeks’ gestation. Raw data values were converted to multiples of the median (MoM). PlGF and sFlt-1 values among platforms were highly correlated (p < 0.001). There was significant variation in raw data values for PlGF and sFlt-1 among platforms, eliminated following conversion to MoM. When directly comparing raw data values among platforms, platform-specific reference ranges values should be used. MoM values were equivalent among platforms, allowing direct inter-assay result comparison.
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Placenta
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86
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Biochemistry and cell biology
Clinical sciences
Science & Technology
Life Sciences & Biomedicine
Developmental Biology
Obstetrics & Gynecology
Reproductive Biology
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Black, C; Al-Amin, A; Rolnik, DL; Kane, SC; Stolarek, C; White, A; Costa, FDS; Brennecke, S, Midpregnancy testing for soluble fms-like tyrosine kinase 1 (sFlt-1) and placental growth factor (PlGF): An inter-assay comparison of three automated immunoassay platforms, Placenta, 2019, 86, pp. 11-14