Glycoproteomics technologies in glycobiotechnology

No Thumbnail Available
File version
Author(s)
Alagesan, Kathirvel
Hoffmann, Marcus
Rapp, Erdmann
Kolarich, Daniel
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)

Rapp, E

Reichl, U

Date
2021
Size
File type(s)
Location
License
Abstract

Glycosylation is a key factor determining the pharmacological properties of biotherapeutics, including their stability, solubility, bioavailability, pharmacokinetics, and immunogenicity. As such, comprehensive information about glycosylation of biotherapeutics is critical to demonstrate similarity. Regulatory agencies also require extensive documentation of the comprehensive analyses of glycosylation-related critical quality attributes (CQAs) during the development, manufacturing, and release of biosimilars. Mass spectrometry has catalysed tremendous advancements in the characterisation of glycosylation CQAs of biotherapeutics. Here we provide a perspective overview on the MS-based technologies relevant for biotherapeutic product characterisation with an emphasis on the recent developments that allow determination of glycosylation features such as site of glycosylation, sialic acid linkage, glycan structure, and content.

Journal Title
Conference Title
Book Title

Advances in Glycobiotechnology

Edition
Volume

175

Issue
Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement
Item Access Status
Note
Access the data
Related item(s)
Subject

Glycobiology

Biochemistry and cell biology

Glycoconjugates

Proteomics and intermolecular interactions (excl. medical proteomics)

Medical biochemistry - proteins and peptides (incl. medical proteomics)

Analytical biochemistry

Pharmacology and pharmaceutical sciences not elsewhere classified

Analytics

Biochemistry & Molecular Biology

Biopharmaceuticals

Biotechnology & Applied Microbiology

capillary-electrophoresis

Persistent link to this record
Citation

Alagesan, K; Hoffmann, M; Rapp, E; Kolarich, D, Glycoproteomics Technologies in Glycobiotechnology, Advances in Glycobiotechnology, 2021, 175, pp. 413-434

Collections