Glycoproteomics technologies in glycobiotechnology
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Hoffmann, Marcus
Rapp, Erdmann
Kolarich, Daniel
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Rapp, E
Reichl, U
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Abstract
Glycosylation is a key factor determining the pharmacological properties of biotherapeutics, including their stability, solubility, bioavailability, pharmacokinetics, and immunogenicity. As such, comprehensive information about glycosylation of biotherapeutics is critical to demonstrate similarity. Regulatory agencies also require extensive documentation of the comprehensive analyses of glycosylation-related critical quality attributes (CQAs) during the development, manufacturing, and release of biosimilars. Mass spectrometry has catalysed tremendous advancements in the characterisation of glycosylation CQAs of biotherapeutics. Here we provide a perspective overview on the MS-based technologies relevant for biotherapeutic product characterisation with an emphasis on the recent developments that allow determination of glycosylation features such as site of glycosylation, sialic acid linkage, glycan structure, and content.
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Advances in Glycobiotechnology
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175
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Subject
Glycobiology
Biochemistry and cell biology
Glycoconjugates
Proteomics and intermolecular interactions (excl. medical proteomics)
Medical biochemistry - proteins and peptides (incl. medical proteomics)
Analytical biochemistry
Pharmacology and pharmaceutical sciences not elsewhere classified
Analytics
Biochemistry & Molecular Biology
Biopharmaceuticals
Biotechnology & Applied Microbiology
capillary-electrophoresis
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Alagesan, K; Hoffmann, M; Rapp, E; Kolarich, D, Glycoproteomics Technologies in Glycobiotechnology, Advances in Glycobiotechnology, 2021, 175, pp. 413-434