A Review of the Advantages, Disadvantages and Limitations of Chemotaxis Assays for Campylobacter spp.

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Elgamoudi, Bassam A
Korolik, Victoria
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2022
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https://creativecommons.org/licenses/by/4.0/
Abstract

Reproducible qualitative and quantitative assessment of bacterial chemotactic motility, particularly in response to chemorepellent effectors, is experimentally challenging. Here we compare several established chemotaxis assays currently used to investigate Campylobacter jejuni chemotaxis, with the aim of improving the correlation between different studies and establishing the best practices. We compare the methodologies of capillary, agar, and chamber-based assays, and discuss critical technical points, in terms of reproducibility, accuracy, and the advantages and limitations of each.

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International Journal of Molecular Sciences
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23
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3
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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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Genetics
Other biological sciences
Other chemical sciences
Biochemistry and cell biology
Microbiology
Medicinal and biomolecular chemistry
Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemistry & Molecular Biology
Chemistry, Multidisciplinary
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Elgamoudi, BA; Korolik, V, A Review of the Advantages, Disadvantages and Limitations of Chemotaxis Assays for Campylobacter spp., International Journal of Molecular Sciences, 2022, 23 (3), pp. 1576
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