Further evidence for biological effects resulting from ionizing radiation doses in the diagnostic X-ray range

No Thumbnail Available
File version
Author(s)
Anoopkumar-Dukie, S
McMahon, A
Allshire, A
Conere, TJ
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
2005
Size
File type(s)
Location
License
Abstract

In this study we assessed cell response to ionizing radiation doses in the diagnostic X-ray range, using metabolic performance as the biological end point. HeLa cells were irradiated between 3.5 mGy (standard deviation (SD=2.5%) and 55.1 mGy (SD=0.9%) with 110 kV X-rays, and after 7 days metabolic capacity of cultures was measured spectrophotometrically with resazurin. Cells receiving 7.5 mGy or greater showed impaired proliferation. At 7.5 mGy this effect was prevented by nitric oxide synthase inhibitor N G-monomethyl-L-arginine but not by ascorbic acid or p38 MAPK inhibitor SB203580. We conclude that HeLa cell proliferation is adversely affected by doses within the range of some radiological examinations, through a mechanism involving nitric oxide.

Journal Title

British Journal of Radiology

Conference Title
Book Title
Edition
Volume

78

Issue

928

Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement
Item Access Status
Note
Access the data
Related item(s)
Subject

Clinical sciences

Clinical sciences not elsewhere classified

Persistent link to this record
Citation
Collections