Aims: The purpose of this study was to assess Emirati university students’ knowledge of physical activity guidelines and perceptions of the benefits of physical activity for mental health. Method: A random sample (n = 628) completed an online questionnaire assessing the recommended amount of physical activity for good health (min/week) and to what extent exercise can help improve wellbeing, and manage depression and anxiety/stress (5-point Likert-type scale). Results: Only 3% of respondents identified 150 min/week and less than half (39%) of the respondents reported an amount within the range of 150–300 min/week. Most respondents believed that physical activity could improve wellbeing (69%), and manage depression (71%) and anxiety/stress (74%). Females were more likely than males to view physical activity as a way to manage depression (p = .003), and anxiety/stress (p = .002). Conclusion: More work may be required to promote awareness of physical activity guidelines in this population, and males’ awareness of mental health benefits of physical activity.