Hydrogels with Cell Adhesion Peptide-Decorated Channel Walls for Cell Guidance

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Wang, Shuang
Sarwat, Mariah
Wang, Peng
Surrao, Denver C
Harkin, Damien G
St John, James A
Bolle, Eleonore CL
Forget, Aurelien
Dalton, Paul D
Dargaville, Tim R
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2020
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http://creativecommons.org/licenses/by/4.0/
Abstract

A method is reported for making hollow channels within hydrogels decorated with cell-adhesion peptides exclusively at the channel surface. Sacrificial fibers of different diameters are used to introduce channels within poly(ethylene glycol) hydrogels crosslinked with maleimide-thiol chemistry, which are backfilled with a cysteine-containing peptide solution which is conjugated to the lumen with good spatial efficiency. This allows for peptide patterning in only the areas of the hydrogel where they are needed when used as cell-guides, reducing the amount of required peptide 20-fold when compared to bulk functionalization. The power of this approach is highlighted by successfully using these patterned hydrogels without active perfusion to guide fibroblasts and olfactory ensheathing cells-the latter having unique potential in neural repair therapies.

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Macromolecular Rapid Communications
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© 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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Chemical sciences
Engineering
Science & Technology
Physical Sciences
Polymer Science
3D printing
cell guidance
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Wang, S; Sarwat, M; Wang, P; Surrao, DC; Harkin, DG; St John, JA; Bolle, ECL; Forget, A; Dalton, PD; Dargaville, TR, Hydrogels with Cell Adhesion Peptide-Decorated Channel Walls for Cell Guidance, Macromolecular Rapid Communications, 2020, 41 (15), pp. 2000295
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