Efficacy of Alum-Adjuvanted Peptide and Carbohydrate Conjugate Vaccine Candidates against Group A Streptococcus Pharyngeal Infection in a Non-Human Primate Model

Loading...
Thumbnail Image
File version

Version of Record (VoR)

Author(s)
Rivera-Hernandez, Tania
Carnathan, Diane G
Richter, Johanna
Marchant, Patrick
Cork, Amanda J
Elangovan, Gayathiri
Henningham, Anna
Cole, Jason N
Choudhury, Biswa
Moyle, Peter M
Toth, Istvan
Batzloff, Michael R
Good, Michael F
Agarwal, Paresh
Kapoor, Neeraj
et al.
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
2024
Size
File type(s)
Location
Abstract

Vaccine development against group A Streptococcus (GAS) has gained traction in the last decade, fuelled by recognition of the significant worldwide burden of the disease. Several vaccine candidates are currently being evaluated in preclinical and early clinical studies. Here, we investigate two conjugate vaccine candidates that have shown promise in mouse models of infection. Two antigens, the J8 peptide from the conserved C-terminal end of the M protein, and the group A carbohydrate lacking N-acetylglucosamine side chain (ΔGAC) were each conjugated to arginine deiminase (ADI), an anchorless surface protein from GAS. Both conjugate vaccine candidates combined with alum adjuvant were tested in a non-human primate (NHP) model of pharyngeal infection. High antibody titres were detected against J8 and ADI antigens, while high background antibody titres in NHP sera hindered accurate quantification of ΔGAC-specific antibodies. The severity of pharyngitis and tonsillitis signs, as well as the level of GAS colonisation, showed no significant differences in NHPs immunised with either conjugate vaccine candidate compared to NHPs in the negative control group.

Journal Title

Vaccines (Basel)

Conference Title
Book Title
Edition
Volume

12

Issue

4

Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement

Copyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Item Access Status
Note
Access the data
Related item(s)
Subject

Clinical sciences

Immunology

Medical microbiology

Persistent link to this record
Citation

Rivera-Hernandez, T; Carnathan, DG; Richter, J; Marchant, P; Cork, AJ; Elangovan, G; Henningham, A; Cole, JN; Choudhury, B; Moyle, PM; Toth, I; Batzloff, MR; Good, MF; Agarwal, P; Kapoor, N; et al., Efficacy of Alum-Adjuvanted Peptide and Carbohydrate Conjugate Vaccine Candidates against Group A Streptococcus Pharyngeal Infection in a Non-Human Primate Model, Vaccines (Basel), 2024, 12 (4), pp. 382

Collections