Efficacy of Alum-Adjuvanted Peptide and Carbohydrate Conjugate Vaccine Candidates against Group A Streptococcus Pharyngeal Infection in a Non-Human Primate Model

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Rivera-Hernandez, Tania
Carnathan, Diane G
Richter, Johanna
Marchant, Patrick
Cork, Amanda J
Elangovan, Gayathiri
Henningham, Anna
Cole, Jason N
Choudhury, Biswa
Moyle, Peter M
Toth, Istvan
Batzloff, Michael R
Good, Michael F
Agarwal, Paresh
Kapoor, Neeraj
et al.
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2024
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https://creativecommons.org/licenses/by/4.0/
Abstract

Vaccine development against group A Streptococcus (GAS) has gained traction in the last decade, fuelled by recognition of the significant worldwide burden of the disease. Several vaccine candidates are currently being evaluated in preclinical and early clinical studies. Here, we investigate two conjugate vaccine candidates that have shown promise in mouse models of infection. Two antigens, the J8 peptide from the conserved C-terminal end of the M protein, and the group A carbohydrate lacking N-acetylglucosamine side chain (ΔGAC) were each conjugated to arginine deiminase (ADI), an anchorless surface protein from GAS. Both conjugate vaccine candidates combined with alum adjuvant were tested in a non-human primate (NHP) model of pharyngeal infection. High antibody titres were detected against J8 and ADI antigens, while high background antibody titres in NHP sera hindered accurate quantification of ΔGAC-specific antibodies. The severity of pharyngitis and tonsillitis signs, as well as the level of GAS colonisation, showed no significant differences in NHPs immunised with either conjugate vaccine candidate compared to NHPs in the negative control group.

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Vaccines (Basel)
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12
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4
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Copyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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Clinical sciences
Immunology
Medical microbiology
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Rivera-Hernandez, T; Carnathan, DG; Richter, J; Marchant, P; Cork, AJ; Elangovan, G; Henningham, A; Cole, JN; Choudhury, B; Moyle, PM; Toth, I; Batzloff, MR; Good, MF; Agarwal, P; Kapoor, N; et al., Efficacy of Alum-Adjuvanted Peptide and Carbohydrate Conjugate Vaccine Candidates against Group A Streptococcus Pharyngeal Infection in a Non-Human Primate Model, Vaccines (Basel), 2024, 12 (4), pp. 382
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