Green turtles foraging in coastal areas are exposed to land-based chemical pollutants that accumulate in the habitats to which they show high site fidelity. However, prior to coastal recruitment, they may be exposed to a different range of chemical threats. The recent development of species-specific in vitro bioassays for marine turtles allows for an effect-based assessment of toxicological endpoints. Blood was collected from green turtles of two life-stages, ‘recent recruits’ and ‘coastal residents’, in Hervey Bay and Moreton Bay. Organic contaminants were extracted from blood using the QuEChERS method, and cytotoxicity of the extracts measured in green turtle skin cells. Although not statistically significant, extracts from ‘coastal residents’ exhibited greater mean toxicity compared to ‘recent recruits’, possibly indicative of increased chemical accumulation from coastal habitat exposure. The bioassay results also indicated that turtles foraging in Hervey Bay are at greater risk of chemical exposure than those foraging in Moreton Bay.