Application of Autologous Platelet-Rich Plasma on Tooth Extraction Site Prevents Occurence of Medication-Related Osteonecrosis of the Jaws in Rats
File version
Version of Record (VoR)
Author(s)
de Mello-Neto, Joao Martins
Ventura dos Santos, Fernanda Furuse
Ferreira, Leticia Chaves
Statkievicz, Cristian
Angelo Cintra, Luciano Tavares
Mardegan Issa, Joao Paulo
Menegati Dornelles, Rita Cassia
de Almeida, Juliano Milanezi
Hitomi Nagata, Maria Jose
Garcia, Valdir Gouveia
Theodoro, Leticia Helena
Casatti, Claudio Aparecido
Ervolino, Edilson
Griffith University Author(s)
Primary Supervisor
Other Supervisors
Editor(s)
Date
Size
File type(s)
Location
Abstract
This study evaluated the effects of local application of autologous platelet-rich plasma (PRP) on the tooth extraction site of rats presenting the main risk factors for medication-related osteonecrosis of the jaw (MRONJ). For seven weeks, senile rats were submitted to systemic treatment with vehicle (VEH and VEH-PRP) or 100 μg/Kg of zoledronate (ZOL and ZOL-PRP) every three days. After three weeks, the first lower molar was extracted. VEH-PRP and ZOL-PRP received PRP at the tooth extraction site. Euthanasia was performed at 28 days postoperatively. Clinical, histopathological, histometric and immunohistochemical analyses were carried out in histological sections from the tooth extraction site. ZOL showed lower percentage of newly formed bone tissue (NFBT), higher percentage of non-vital bone tissue (NVBT), as well as higher immunolabeling for TNFα and IL-1β. In addition, ZOL presented lower immunolabeling for PCNA, VEGF, BMP2/4, OCN and TRAP. VEH and ZOL-PRP showed improvement in the tooth extraction site wound healing and comparable percentage of NFBT, VEGF, BMP2/4 and OCN. Local application of autologous PRP proved a viable preventive therapy, which is safe and effective to restore tissue repair capacity of the tooth extraction site and prevent the occurrence of MRONJ following tooth extraction.
Journal Title
Scientific Reports
Conference Title
Book Title
Edition
Volume
9
Issue
1
Thesis Type
Degree Program
School
Publisher link
Patent number
Funder(s)
Grant identifier(s)
Rights Statement
Rights Statement
© The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Item Access Status
Note
Access the data
Related item(s)
Subject
Dentistry
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
BISPHOSPHONATE-RELATED OSTEONECROSIS
ZOLEDRONIC ACID
Persistent link to this record
Citation
Toro, LF; de Mello-Neto, JM; Ventura dos Santos, FF; Ferreira, LC; Statkievicz, C; Angelo Cintra, LT; Mardegan Issa, JP; Menegati Dornelles, RC; de Almeida, JM; Hitomi Nagata, MJ; Garcia, VG; Theodoro, LH; Casatti, CA; Ervolino, E, Application of Autologous Platelet-Rich Plasma on Tooth Extraction Site Prevents Occurence of Medication-Related Osteonecrosis of the Jaws in Rats, Scientific Reports, 2019, 9 (1), pp. 22