Currently, diabetes is a prevalent ailment worldwide. Insufficient insulin leads to an inability to transform glucose into energy, resulting in increased amounts of glucose in human body. Acute diabetes treatment and follow-up rely on urine glucose detection since it provides a non-invasive, reasonably priced means to assess glucose levels and regulate the progression of the disease. This article presents the development of a circular shape core and semi-circular trapezoid air holes biosensor PCF to assess the glucose concentration in urine inside the THz band. With matching RI's of 1.335, 1.336, 1.337, 1.338, 1.341, and 1.347, glucose quantity in bio-sample spans 0 to 15 mg/dl (for as usual people) and 0.625 g/dL, 1.25 g/dL, 2.5 g/dL, 5 g/dL, and 10 g/dL (for diabetic patients) and achieve highest sensitivity is 99.12 %, EML loss 0.0021 cm−1, 2.24× 10−13 dB/m for 10 g/dl. The innovative design of this device guarantees precise and non-intrusive monitoring of the various stages of diabetes, allowing for timely and efficient control of the condition. This revolutionary sensor improves patient care and facilitates early intervention by offering a dependable and cost-efficient alternative to conventional approaches. Timely identification aids in averting complications and enhances patient prognosis.