The impacts of ammonium-based N ($$ {/text{NH}}^{ + }{4} - {/text{N}} $$) addition on soil nitrification and acidification were investigated in terms of kinetic mechanisms and major factors controlling these soil processes for terrestrial ecosystems in subtropical China. Soil samples were collected from an upland soil derived from a sandstone parent (SU), a brush-land soil from a granite (GB), and a forest soil from a quaternary red earth (QF) in a typical subtropical region of China. The samples were incubated at 30àwith soil moisture content of 60% water holding capacity (WHC) for 35 days, after adding ammonium sulphate, urea, and ammonium bicarbonate at rates of 0, 100, and 250 mg N kg-1, respectively. Nitrification in SU soil (pH 6.27) followed a first-order reaction model (P?<?0.001). Addition of ammonium sulphate, urea and ammonium bicarbonate significantly (P?<?0.05) stimulated nitrification. As a result, the soil was significantly acidified (P?<?0.05) and the soil pH at the end of incubation decreased with increasing N addition. In contrast, nitrification in QF (pH 4.46) and GB (pH 4.82) soils followed a zero-order reaction model (P?<?0.001) and hence the addition of $$ {/text{NH}}^{ + }{4} $$ did not directly affect soil nitrification. However, the chemical input directly changed initial pH of GB and QF soils, resulting in either a decrease or an increase in $$ {/text{NO}}^{ - }{3} $$ production, dependent on the impact of the chemicals applied. At the end of incubation, the pH of QF and GB soils was significantly higher (P?<?0.05) in treatments with $$ {/text{NH}}^{ + }{4} $$-input than without $$ {/text{NH}}^{ + }{4} $$-input. These results indicated that for some acid soils nitrification was not controlled by available $$ {/text{NH}}^{ + }{4} $$-N and that $$ {/text{NH}}^{ + }_{4} $$-N-input was not necessary to stimulate soil nitrification. And so no acceleration of soil acidification occurred. In order to characterize nitrification intensity in these humid soils and its effect on acidification, nitrification without N-amendment is a better indicator than with N-amendment.