For patients suffering from end-stage heart failure (HF), heart transplant (HTx) can often be the only viable option for treatment. However, the availability of hearts and success of surgery is a requisite for decreasing overall mortality. It has long been known that diet has an influence on overall cardiovascular health and wellbeing. Hence, this study observes two factors effected by diet; (1) diabetes, (2) TMA lyase inhibition, a therapeutic acting within the gut to reduce trimethylamine N-oxide (TMAO) – a potential dietary marker of disease. As donor hearts aren’t always in ideal health, it is important to understand the effects of these factors on the outcomes of cold static storage (CSS) for HTx. [...]