HIV-1 dynamically trap specific lipids during assembly in living T cells

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Favard, C
Chojnacki, J
Merida, P
Yandrapalli, N
Mak, J
Eggeling, C
Muriaux, D
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2019
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Madrid, Spain

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Abstract

HIV-1 Gag protein self-assembles at the plasma membrane of infected cells for viral particle formation. Gag targets lipids, mainly the phosphatidylinositol (4,5) bisphosphate, at the inner leaflet of this membrane. We recently shown that Gag alone was sufficient to trap PI(4,5)P2 in biomimetic membranes[1]. In this study, we address the question whether Gag is able to trap PI(4,5)P2 and/or other lipids during HIV-1 assembly in the living host CD4+ T lymphocytes. Here, we determine lipid dynamics within and away from HIV-1 assembly sites using super-resolution STED microscopy coupled with scanning Fluorescence Correlation Spectroscopy. Analysis of HIV-1 infected cells revealed that, upon virus assembly, HIV-1 is able to specifically trap PI(4,5)P2, and cholesterol, but not phosphatidylethanolamine nor sphingomyelin. Furthermore, our data show that Gag is the main driving force to restrict mobility of PI(4,5)P2 and cholesterol at the cell plasma membrane. This is first direct evidence showing that HIV-1 creates its own specific lipid environment by selectively recruiting PI(4,5)P2 and cholesterol, instead of targeting pre-existing ones as it was previously suggested.

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European Biophysics Journal

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48

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1 Supplement

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Other physical sciences

Biochemistry and cell biology

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Life Sciences & Biomedicine

Biophysics

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Favard, C; Chojnacki, J; Merida, P; Yandrapalli, N; Mak, J; Eggeling, C; Muriaux, D, HIV-1 dynamically trap specific lipids during assembly in living T cells, European Biophysics Journal, 2019, 48, pp. S189-S189