Human neutrophils secrete bioactive paucimannosidic proteins from azurophilic granules into pathogen-infected sputum
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Venkatakrishnan, Vignesh
Loke, Ian
Laurini, Christine
Diestel, Simone
Parker, Benjamin L.
Packer, Nicolle H.
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Abstract
Background: Protein paucimannosylation is considered an important invertebrate- and plant-specific glycoepitope.
Results: Azurophilic granule-specific human neutrophil proteins from pathogen-infected sputum displayed significant core-fucosylated paucimannosylation generated by maturation- and granule-specific β-hexosaminidase A and were preferentially secreted from non-lysosomal origins into sputum upon P. aeruginosa stimulation.
Conclusion: Human neutrophils produce, store, and selectively secrete bioactive paucimannosidic proteins.
Significance: This work will aid in understanding the function(s) of human paucimannosylation in glycoimmunology.
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Journal of Biological Chemistry
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290
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14
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This research was originally published in Journal of Biological Chemistry (JBC). Thaysen-Andersen et al, Human neutrophils secrete bioactive paucimannosidic proteins from azurophilic granules into pathogen-infected sputum, Journal of Biological Chemistry (JBC), 2015; 290(14): 8789-8802. Copyright the American Society for Biochemistry and Molecular Biology. Reproduced in accordance with the copyright policy of the publisher. Please refer to the journal's website for access to the definitve version.
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Chemical sciences
Biological sciences
Biochemistry and cell biology not elsewhere classified
Biomedical and clinical sciences