Introduction Ultrasound is a quick, safe, and non-invasive imaging method that can be used to measure skin thickness in pathological cutaneous conditions in clinical and research settings. Despite widespread use, there exists a lack of standardisation and reporting of ultrasound skin thickness measurement methods, which makes between-studies comparisons difficult. To address this, we present a scoping review protocol, which aims to determine what is and is not known about the measurement of skin and scar thickness using ultrasound in people with traumatic scars. Methods and analysis The Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews guidelines and Joanna Briggs Institute scoping review methodology will be used to guide this review. Electronic database searching will be conducted in Ovid MEDLINE, Embase, Cumulative Index of Nursing and Allied Health Literature and Web of Science. No date limit will be imposed on the database searches. Records will be supplemented with searches of reference lists of included studies and grey literature in OpenGrey and Google Advanced. Screening will be conducted by two independent reviewers, and studies where ultrasound is used to measure skin and scar thickness in people with traumatic scars will be included. Data extraction will include ultrasound methods (eg, transducer orientation), psychometric properties (eg, reliability, measurement error), health service and implementation outcomes (eg, feasibility, acceptability) and factors influencing ultrasound measurement of skin thickness (eg, body location, age). Ethics and dissemination Ethical approval is not required for this investigation, as published literature will form the basis of the review. The review will be published in a peer-reviewed scientific journal and is expected to result in the development of the first evidence-based and consensus-based methodological guideline for skin thickness measurement by ultrasound.