Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq

Thumbnail Image
File version

Version of Record (VoR)

He, Bing
Liu, Shuning
Wang, Yuanyuan
Xu, Mengxin
Cai, Wei
Liu, Jia
Bai, Wendi
Ye, Shupei
Ma, Yong
Hu, Hengrui
Meng, Huicui
Sun, Tao
Li, Yanling
Yang, Yuedong
et al.
Griffith University Author(s)
Primary Supervisor
Other Supervisors
File type(s)

B cell response plays a critical role against SARS-CoV-2 infection. However, little is known about the diversity and frequency of the paired SARS-CoV-2 antigen-specific BCR repertoire after SARS-CoV-2 infection. Here, we performed single-cell RNA sequencing and VDJ sequencing using the memory and plasma B cells isolated from five convalescent COVID-19 patients, and analyzed the spectrum and transcriptional heterogeneity of antibody immune responses. Via linking BCR to antigen specificity through sequencing (LIBRA-seq), we identified a distinct activated memory B cell subgroup (CD11chigh CD95high) had a higher proportion of SARS-CoV-2 antigen-labeled cells compared with memory B cells. Our results revealed the diversity of paired BCR repertoire and the non-stochastic pairing of SARS-CoV-2 antigen-specific immunoglobulin heavy and light chains after SARS-CoV-2 infection. The public antibody clonotypes were shared by distinct convalescent individuals. Moreover, several antibodies isolated by LIBRA-seq showed high binding affinity against SARS-CoV-2 receptor-binding domain (RBD) or nucleoprotein (NP) via ELISA assay. Two RBD-reactive antibodies C14646P3S and C2767P3S isolated by LIBRA-seq exhibited high neutralizing activities against both pseudotyped and authentic SARS-CoV-2 viruses in vitro. Our study provides fundamental insights into B cell response following SARS-CoV-2 infection at the single-cell level.

Journal Title

Signal Transduct Target Ther

Conference Title
Book Title




Thesis Type
Degree Program
Publisher link
Patent number
Grant identifier(s)
Rights Statement
Rights Statement

© The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Item Access Status
Access the data
Related item(s)

Clinical sciences

Medical microbiology

Persistent link to this record

He, B; Liu, S; Wang, Y; Xu, M; Cai, W; Liu, J; Bai, W; Ye, S; Ma, Y; Hu, H; Meng, H; Sun, T; Li, Y; Yang, Y; et al., Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq., Signal Transduct Target Ther, 2021, 6 (1), pp. 195-